Table 3.
Compound | 5′-O group | 5′-C group | 3 J H1′-H2′ (Hz) | % C3′ endo a |
---|---|---|---|---|
Guanosine | Triphosphate | H | 6.0 | ∼40 |
30 | Triphosphate | (R)-methyl | 7.0 | ∼30 |
34 | Triphosphate | (S)-methyl | 6.1 | ∼40 |
aThe percent of molecules in the C3′-endo conformation was calculated as (100 – (10 × 3JH1′–H2′)) as previously described (52), where 3JH1′–H2′ is the 1H NMR coupling constant of the sugar H1′ and H2′ protons.