Figure 10.

Structural insights into DNA recognition by CAjan phage DpdA. (A) Homology model of CAjan phage DpdA (cyan) superposed on the catalytic domain of P. horikoshii arcTGT (brown, PDB ID 1IT8) viewed down the TIM barrel. (B) Close up of the superposed active sites showing preQ₀ as seen bound to arcTGT. DpdA residues are labeled. (C) Docking model of dsDNA harboring a flipped G onto CAjan phage DpdA showing the putative DNA binding mode to the positively charged groove on the protein surface. The protein is shown in electrostatic surface potential representation. The active site in (A) and (C) is indicated with an asterisk.