Skip to main content
. 2020 Sep 22;48(18):10226–10240. doi: 10.1093/nar/gkaa736

Figure 4.

Figure 4.

ETO2 antagonizes LDB1-complex enhancer activity and chromatin looping between the β-globin locus LCR enhancer and active genes. (A) Representative screenshots of RNA-seq, TAL1, LDB1 and ETO2 ChIPmentation signals in the β-globin gene locus. (B−E) ChIP-qPCR of ETO2, LDB1, MED1 and Pol II occupancy in the LCR HS1-HS4 and γ-globin promoter for WT and ETO2 KO K562 cells. (F) Chromosome conformation capture (3C) indicates LCR/globin gene interaction frequency in WT, ETO2-KO and ETO2-overexpressing cells. LCR serves as anchor. Ggamma primer does not distinguish between A/Ggamma genes and signal represenrs the interaction frequency for both genes. Error bars indicate SD. N = 3 biological replicates. *P< 0.05, **P< 0.01, ***P< 0.001 by two-tailed Student's t-test.