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. 2020 Sep 22;48(18):10226–10240. doi: 10.1093/nar/gkaa736

Figure 5.

Figure 5.

ETO2 occupancy affects the enrichment of nucleosome remodeling and deacetylating NuRD complex in the β-globin locus. (A) Co-IP analysis of reciprocal interactions between ETO2 and CHD4. (B−E) ChIP-qPCR for CHD4, HDAC1, MTA2 and RBBP4 occupancy at the LCR HSs and γ-globin gene in WT and ETO2-KO cells. (F) MNase-qPCR showing sensitivity to MNase digestion of the globin gene locus in WT and ETO2-KO cells. (G, H) ChIP-qPCR for H3K9ac and H3K27ac in LCR HSs and γ-globin gene in WT and ETO2-KO cells. (I) Model depicting reduced ETO2/LDB1 ratio, elevated H3K27ac and H3K9ac modification and γ-globin looping to the LCR after loss of ETO2. Error bars indicate SD. N = 3 biological replicates. *P< 0.05, **P< 0.01 by two-tailed Student's t-test.