Figure 1.

The htb Mutant Produces Fruits with Defective Fruit Shape and Reduced Anisotropic Cell Growth

(A–C) Fruit morphology of WT (A), htb-1 (B), and rescue line of htb-1 transformed with pHTB:HTB:GFP (C) at stage 17.

(D) Shoulder index measurements of fruits from WT, htb-1, and htb-1^re (pHTB:HTB:GFP htb-1) plants.

(E–G) Scanning electron microscopy (SEM) images of fruits from WT at developmental stages 12 (E), 13 (F), and 14 (G), showing fruit-shoulder growth after pollination.

(H–J) SEM images of fruits from htb-1 at stages 12 (H), 13 (I), and 14 (J), showing compromised development of the fruit shoulders.

(K–N) Time-lapse live imaging of developing fruits from stages 12 to 13 and 13 to 14 in WT (K and M) and htb-1 mutant (L and N). Cells are outlined by RFP signal of the clonal sectors derived from heat-shock treatment of pHS:CRE/BOB-lox line. The heatmaps represent the anisotropy (K and L) and the overall cell area ratio (M and N).

Scale bars, (A–C) 5 mm; (E–N) 100 μm. Error bars in (D) represent SD of 30 individual fruits. ^∗∗p < 0.01 (Student’s t test). See also Figure S1.