Figure 2.

Identification of EXPA-Binding Transcription Factors

(A) Schematics of genomic regions of embryo-induced EXPA genes. 3′ UTR regions are colored in purple, and exons are colored in green. The numbers below the gray lines indicate relative positions upstream of the transcription start site (TSS). Promoter bait fragments used in the Y1h assay are labeled with the numbers 1 to 4, consisting of ~300- to ~400-bp sequences with 100-bp overlaps between adjacent fragments.

(B) Pie chart showing the categorization of transcription factor (TF) families included in the Y1h library screened (Data S1B).

(C) Pie chart showing the percentage of TFs by family that bound to EXPA promoters in yeast (Data S1C).

(D) Heatmaps showing the number of TFs that bound to the overlapping promoter fragments of EXPA genes.

(E) Interaction between TFs and EXPA promoter fragments in Y1h assays. Node color indicates TF family after the legend on the right.

See also Figures S2–S4.