Figure 3.

Tip60 acetyltransferase activity is critical for HSC function. (A) The experimental scheme for in vivo repopulating assays: Tip60^Δ/Δ (Tip60^f/f; Vav-iCre) LSK cells at E13.5 (CD45.2⁺) were transduced with TIP60^wt, TIP60^mut (supplemental Figure 4B), and EV plasmid retrovirally, and transplanted into lethally irradiated recipient mice (CD45.1⁺), along with congenic WBM cells (CD45.1⁺CD45.2⁺). Transduction of plasmid DNA was verified by Sanger sequencing of the PCR-amplified genomic products. (B) Chimerism of recipient PB was monitored at different times for 16 weeks after the transplantation. Percentages of donor cells in total (all nucleated cell), myeloid (Gr1⁺Mac1⁺), B cells (CD19⁺B220⁺), and T cells (CD3⁺CD4⁺) of recipient PB are shown. Values are presented as means ± standard error of the mean. Statistical analyses were performed vs EV. *P < .05, ns; not significant.