Table 2.

Least squares means of plasma phospholipid fatty acid (FA) concentrations on average d 1, 2, 4, and 7

Plasma phospholipid FA1 (g/100 g)	Treatment2				L3	Q4	C5	Overall P-value6
	CON	FF30	FF60	FF120
C18:2 (n-6; LA)	18.2	19.9	21.0	19.7	0.22	0.04	0.87	0.12
C18:3 (n-3; ALA)	0.35	0.88	1.13	1.27	<0.001	<0.001	0.13	<0.001
C20:2 (n-6)	0.24	0.24	0.24	0.21	0.19	0.48	0.99	0.51
C20:3 (n-6)	1.74	1.58	1.37	1.17	0.004	0.63	0.79	0.03
C20:4 (n-6; ARA)	4.62	4.37	4.16	4.27	0.63	0.65	0.93	0.92
C20:5 (n-3; EPA)	0.42	0.76	1.29	1.75	<0.001	0.02	0.65	<0.001
C22:4 (n-6)	0.34	0.33	0.24	0.24	0.17	0.68	0.47	0.44
C22:5 (n-6)	0.11	0.12	0.08	0.08	0.23	0.64	0.35	0.46
C22:5 (n-3)	1.36	1.36	1.34	1.55	0.11	0.29	0.70	0.28
C22:6 (n-3; DHA)	1.22	1.56	1.71	2.16	<0.001	0.65	0.52	<0.001
Σ SFA	38.9	39.6	39.8	39.5	0.11	0.03	0.69	0.07
Σ MUFA	23.8	22.9	21.1	21.5	0.008	0.08	0.30	0.02
Σ PUFA	26.3	28.5	29.6	29.8	<0.001	0.02	0.85	0.002
Σ n-3 FA	3.44	4.59	5.64	6.95	<0.001	0.02	0.94	<0.001
Σ n-6 FA	25.3	26.7	27.2	25.8	0.83	0.06	0.98	0.27
Σ n-6 FA:n-3 FA	6.99	5.47	4.66	3.38	<0.001	0.42	0.68	<0.001

¹Plasma phospholipid FA: linoleic acid (LA), α-linolenic acid (ALA), arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA).

²Treatments (n = 6): CON = control, no supplement added to colostrum; FF30 = 30 mL of a 1:1 ratio fish and flaxseed oil blend added to colostrum; FF60 = 60 mL of a 1:1 ratio fish and flaxseed oil blend added to colostrum; FF120 = 120 mL of a 1:1 ratio fish and flaxseed oil blend added to colostrum.

³L = linear polynomial contrast.

⁴Q = quadratic polynomial contrast.

⁵C = cubic polynomial contrast.

⁶Overall P-value = treatment effect P-value.