3.

紫草素对I-10和TCAM-2细胞增殖和凋亡的抑制作用

Shikonin inhibits proliferation and apoptosis of I-10 and TCAM-2 cells. A: The effect of shikonin on the proliferation inhibition of I-10 cells; B: The effect of shikonin on the proliferation inhibition of TCAM-2 cells; C: Transmission electron microscopy was used to detect the effect of shikonin on apoptosis of I- 10 cells (The black arrow indicates the nucleus; the red arrow indicates the mitochondria); D: Transmission electron microscopey was used to detect the effect of shikonin on apoptosis of TCAM-2 cells(The black arrow indicates the nucleus; the red arrow indicates the mitochondria); E: Annexin V-FITC/PI double staining method was used to detect the effect of shikonin on apoptosis of I-10 cells; F: Annexin V-FITC/PI double staining was used to detect the effect of shikonin on apoptosis of I-10 cells; G-J: Western blot evaluation of the expression of mitochondrial apoptosis- related proteins Bax, Bcl- 2, cleaved caspase- 3 in I- 10 cells; K-N: Western blot evaluation of the expression of Bax, Bcl-2 and cleaved caspase-3 in TCAM-2 cells (n=3, ^*P < 0.05, ^**P < 0.01 vs control group.)