Fig. 2.

Determination of the cut-off values for SENA detection (a) and the LoD values with 95% CI for both rRT-PCR (O-Ct, green dots) and SENA (mix-FCratio, black dots) (b) based on the systematic titration assays. All the experimental and analytical details are described in the text. Notice that the SENA negative cut-off was set as mix-FCratio=1.020 in this figure on the basis of the titration assay of the standard RNA templates but was adjusted to 1.068 along with the increase of the clinical applications (Figs. 1 and 4, Supplementary Table 3). LoD with 95% confidence interval was 1.2≤1.6≤2.1 with mix-SENA versus 3.3≤4.0≤6.1 with rRT-PCR.