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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Neuropharmacology. 2020 Aug 8;178:108126. doi: 10.1016/j.neuropharm.2020.108126

Figure 5. Intermittent ethanol increases medium afterhyperpolarization (mAHP) in somatostatin (SST-IN) interneurons in male mice.

Figure 5.

(A) Representative responses to negative current injection steps (−150, −100, −50, 0 pA) in SST-INs from control and IA ethanol mice. Scale bars indicate 10 mV, 200 ms. (B) Minimal differences in SST-IN membrane resistance (Rm) between control and IA ethanol mice (Two-way ANOVA sex x IA ethanol interaction: F1,54 = 3.045, p < 0.09). n/N = 14-16/4-5 cells/mice per group. (C) Representative spike-firing in response to 100-pA (top) and 200-pA (bottom) injections in SST-INs from control (black) and IA ethanol (blue) mice. Scale bars indicate 10 mV, 200 ms. (D) No differences in current-evoked spiking were observed related to IA ethanol in SST-INs (Three-way RM ANOVA). n/N = 15-16/4-5. (E) Representative mAHP in SST-INs from control and IA ethanol mice. mAHP was calculated as the decrease in membrane potential within 1-sec following sustained depolarization and spike-firing. Scale bars indicate 5 mV, 250 ms. (F) SST-INs from male IA ethanol mice displayed a trend towards enhanced mAHP relative to male controls (Two-way ANOVA sex x IA ethanol interaction: F1,54 = 6.513, p < 0.02, #: p < 0.1 Sidak post test). n/N = 14-16/4-5.