Skip to main content
. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Neuropharmacology. 2020 Aug 8;178:108126. doi: 10.1016/j.neuropharm.2020.108126

Figure 7. Intermittent access to ethanol (IA ethanol) decreases excitatory synaptic strength onto somatostatin interneurons (SST-INs).

Figure 7.

(A) Left, Averaged sEPSC from representative experiment. Scale bars indicate 5 pA, 2 ms. Right, Representative spontaneous excitatory postsynaptic current (sEPSC) traces from SST-INs from control (black) and IA ethanol (blue) female mice. Scale bars indicate 10 pA, 100 ms. (B) Decreased sEPSC amplitude was observed in SST-INs from IA ethanol mice relative to controls. (Two-way ANOVA, main effect of IA ethanol). n/N = 12-17/4-5 cells/mice per group. Right, SST-INs from mice given IA ethanol displayed decreased sEPSC frequency relative to controls. (Two-way ANOVA, main effect of IA ethanol). n/N = 12-17/4-5. (C) Representative recordings from paired-pulse ratio (PPR) experiments. Scale bars indicate 20 pA, 25 ms. (D) Sex difference in PPR following IA ethanol (Three-way repeated-measures ANOVA, sex x ISI interaction: F4,156 = 5.736, p < 0.001; IA ethanol x ISI interaction: F4,156 = 2.804, p < 0.03). Left, No group difference in paired-pulse ratio (PPR) between control and IA ethanol female mice across multiple interstimulus intervals (ISI). n/N = 7-9/3, Right, Decreased PPR in SST-INs from male mice with access to IA ethanol (Two-way RM ANOVA, ISI x IA ethanol interaction: F4,84 = 4.6, p < 0.003; main effect of ISI: F4,84 = 24.85, p < 0.0001; main effect of IA ethanol: F1,21 = 6.897, p < 0.02; #: p < 0.1, *: p < 0.05, **: p < 0.01, Sidak post test) n/N = 11-12/4.