| Antibodies |
| pp-ERK2 antibody (T202/Y204) |
Cell Signaling |
Cat#9101 |
| pp-p38 antibody (T180/Y182) |
Cell Signaling |
Cat #9215 |
| pp-ERK5 antibody (Thr218/Tyr220) |
Cell Signaling |
Cat #3371 |
| Phospho-MAPKAPK-2 (T222) |
Cell Signaling |
Cat#3316S |
| Phospho-MAPKAPK-2 (S272) |
Invitrogen |
Cat#PAS-39792 |
| Phospho-MAPKAPK-2 (T334) |
Cell Signaling |
Cat #3007S |
| phospho-RSK1 antibody (T573) |
Cell Signaling |
Cat #9346 |
| phospho-ATF2 (T69/T71) |
Cell Signaling |
Cat #9225 |
| FLAG |
Sigma Aldrich |
Cat #F1804 |
| GAPDH |
Sigma Aldrich |
Cat#G9545 |
| IRDye 680 RD goat anti-Rabbit |
Li-Cor |
Cat#925–32211 |
| IRDye 680 RD goat anti-Mouse |
Li-Cor |
Cat#925–68070 |
| IRDye 800 CW goat anti-Rabbit |
Li-Cor |
Cat#925–32211 |
| IgG HRP-linked antibody anti-rabbit |
Cell Signaling |
Cat#7074S |
| IgG HRP-linked antibody anti-mouse |
Millipore |
Cat #401215 |
| MK2 (total) |
Cell Signaling |
Cat#3042 |
| p38 (total) |
Cell Signaling |
Cat#9228 |
| Bacterial and Virus Strains |
|
E. coli Rosetta(DE3) |
Merck (Novagen) |
Cat#70954–3 |
| Chemicals, Peptides, and Recombinant Proteins |
| TEV protease |
Produced in house |
Reményi Lab |
| Puromycin |
Sigma Aldrich |
Cat#P8833 |
| Doxycycline |
Sigma Aldrich |
Cat#D9891 |
| SB202190 |
Sigma Aldrich |
Cat#S7067 |
| PoA inhibitor |
Synthesized in house |
Reményi Lab |
| PEG 8000 |
Sigma Aldrich |
Cat#P2139 |
| HEPES |
Sigma Aldrich |
Cat#H3375 |
| MPD |
Sigma Aldrich |
Cat#112100 |
| ATP |
Sigma-Aldrich |
Cat#A26209
|
| AMPPNP |
Sigma-Aldrich |
Cat#10102547001 |
| D2O |
Sigma-Aldrich |
Cat#151882 |
| Coelenterazine h |
NanoLight Technology |
Cat#50909–86-9 |
| Lipofectamin 2000 |
ThermoFisher Scientific |
Cat#11668019 |
| Deposited Data |
| pp-p38-MK2 structure with PoA inhibitor |
This paper |
PDB: 6TCA |
| p38-MK2 complex |
White at al. |
PDB: 2OZA |
| p38-MK2 complex with PoA inhibitor |
Cumming et al. |
PDB: 4TYH |
| ERK2-RSK1 complex |
Alexa et al. |
PDB: 4NIF |
| Experimental Models: Cell Lines |
| SF9 cells |
Invitrogen |
Cat#LSB82501 |
| Experimental Models: Organisms/Strains |
| HEK293T |
ATCC |
Cat#CRL-3216 |
| Recombinant DNA |
| pBit1.1N (p38) |
Promega |
Cat#N2014 |
| pBit1.1C (ERK2) |
Promega |
Cat#N2014 |
| pBit1.1C (ERK5) |
Promega |
Cat#N2014 |
| pBit2.1C (MK2) |
Promega |
Cat#N2014 |
| pBit2.1N (RSK) |
Promega |
Cat#N2014 |
| pEBTetD (MKK1EE) |
Bach et al, 2007 |
Reményi Lab |
| pEBTetD (MKK6EE) |
Bach et al, 2007 |
Reményi Lab |
| pEBTetD (MKK7EE) |
Bach et al, 2007 |
Reményi Lab |
| pEBTetD (MKK5DD) |
Bach et al, 2007 |
Reményi Lab |
| pETARA-MK2 (modified pET15b vector) |
Novagen |
Reményi Lab |
| pETARA-RSK1 (modified pET15b vector) |
Novagen |
Reményi Lab |
| PETARA-ERK5 (modified pET15b vector) |
Novagen |
Reményi Lab |
| MG950-p38/λ-phosphatase (modified bicistronic pET15b vector) |
Novagen |
Reményi Lab |
| MG950-ERK2/λ-phosphatase (modified bicistronic pET15b vector) |
Novagen |
Reményi Lab |
| pFastBac-MKK5DD |
ThermoFisher Scientific |
Cat#10360014 |
| MG950-p38/MKK6EE (modified bicistronic pET15b vector) |
Novagen |
Reményi Lab |
| MG950-ERK2/MKK1EE (modified bicistronic pET15b vector) |
Novagen |
Reményi Lab |
| pET28a-BirA |
Addgene |
Cat#20857 |
| Software and Algorithms |
| Origin |
OriginLab |
www.originlab.com |
| Image Studio Lite |
LI-COR |
www.licor.com/bio/image-studio-lite/ |
| Coot |
Emsley et al., 2010 |
www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot |
| Phenix |
Liebschner et al., 2019 |
www.phenix-online.org |
| XDS |
Kabsch, 2010 |
xds.mpimf-heidelberg.mpg.de/ |
| Phaser |
McCoy et al., 2007 |
www.phaser.cimr.cam.ac.uk |
| Aimless |
Evans and Murshudov, 2013 |
www.ccp4.ac.uk/html/aimless.html |
| Modeller |
B. Webb, A. Sali, 2016 |
salilab.org/modeller/ |
| Haddock |
van Zundert et al., 2016 |
haddock.science.uu.nl/services/HADDOCK2.2/ |
| Pymol |
PyMOL Molecular Graphics System
|
pymol.org |
| Atsas package |
Franke et al., 2017 |
www.embl-hamburg.de/biosaxs/software.html |
| Topspin |
Bruker |
www.bruker.com |
| NMRFAM-SPARKY |
Lee et al., 2009 |
nmrfam.wisc.edu/nmrfam-sparky-distribution/ |
| BiaEvaluation software |
GE Healthcare |
https://www.biacore.com |
