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. Author manuscript; available in PMC: 2021 Oct 6.
Published in final edited form as: Cell Metab. 2020 Sep 2;32(4):654–664.e5. doi: 10.1016/j.cmet.2020.08.001

Figure 1. Acute Hepatic DGAT2 KD Induces HIR.

Figure 1.

(A) Hepatic and WAT DGAT2 protein content measured by western blot (top) and its quantification (bottom).

(B) EGP and its suppression by insulin during a hyperinsulinemic-euglycemic clamp in Ctrl vs acute hepatic DGAT2 KD rats.

(C) Levels of insulin-stimulated liver pIRK-Y1162, pAkt-S473, pGSK3β-S9 and pFOXO1-S256 as measured by western blot (top) and with its quantification (bottom).

(D) Liver PKCε translocation from cytosol to membrane as measured by western blot (top) and with its quantification (bottom).

(E) Levels of liver pIRK-T1160 in Ctrl vs acute hepatic DGAT2 KD rats as measured by western blot (top) and with its quantification (bottom).

In all panels, data are the mean±S.E.M. In (A) and (C), n = 6 per group. In (B), n = 8 per group. In (D), n = 5 or 6 per group. In (E), n = 5 per group. *P < 0.05, **P < 0.01 and ***P < 0.001.