KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| DGAT2 | Santa Cruz Biotechnology | Cat#sc-293211 |
| GAPDH | Cell Signaling Technology | Cat#5174 |
| IRK pY1162 | Cell Signaling Technology | Cat#3918 |
| IRK | Cell Signaling Technology | Cat#3025 |
| Akt pS473 | Cell Signaling Technology | Cat#4060 |
| Akt | Cell Signaling Technology | Cat#2920 |
| GSK3β pS9 | Cell Signaling Technology | Cat#5558 |
| GSK3β | Cell Signaling Technology | Cat#12456 |
| FOXO1 pS256 | Cell Signaling Technology | Cat#84192 |
| FOXO1 | Cell Signaling Technology | Cat#2880 |
| PKCε | BD Biosciences | Cat#610086 |
| Na-K ATPase | Abcam | Cat#ab7671 |
| IRK pT1160 | This paper | N/A |
| Calnexin | Abcam | Cat#ab22595 |
| VDAC | Abcam | Cat#ab14734 |
| Perilipin | Cell Signaling Technology | Cat#9349 |
| Bacterial and Virus Strains | ||
| AAV8-TBG-eGFP | Vector Biolabs | SKU#VB1743 |
| AAV8-TBG-rPKCe(A159E) | Vector Biolabs | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Human insulin | Novo Nordisk | NDC 0169-1833-11 |
| [1,2,3,4,5,6,6-2H7] glucose | Cambridge Isotopes | Cat#DLM-2062 |
| [6,6-2H2] glucose | Cambridge Isotopes | Cat#DLM-349 |
| [1,2,3,4,5,6-13C6] glucose | Cambridge Isotopes | Cat#CLM-1396 |
| Somatostatin-14 | Bachem | Cat#H-1490 |
| Somatostatin-28 | Bachem | Cat#H-4955 |
| Amyloglucosidase | Millipore-Sigma | Cat#A7420 |
| Diacylglycerol concentration standard | Indofine Chemical | Cat#32–1902 |
| Ceramide concentration standard | Avanti Polar Lipids | Cat#860517P |
| Critical Commercial Assays | ||
| HR Series NEFA-HR (2) Color Reagent A | Wako | Cat#999–34691 |
| HR Series NEFA-HR (2) Solvent A | Wako | Cat#995–34791 |
| HR Series NEFA-HR (2) Color Reagent B | Wako | Cat#991–34891 |
| HR Series NEFA-HR (2) Solvent B | Wako | Cat#993–35191 |
| NEFA Standard Solution | Wako | Cat#276–76491 |
| Triglyceride-SL reagent | Sekisui | Cat#236–99 |
| Experimental Models: Organisms/Strains | ||
| Sprague-Dawley rats | Charles River | Strain code:400 |
| Oligonucleotides | ||
| MOE control ASO: CCTTCCCTGAAGGTTCCTCC | Ionis Pharmaceuticals | ISIS-141923 |
| DGAT2 ASO: GCATTACCACTCCCATTCTT | Ionis Pharmaceuticals | ISIS-369235 |
| MOE GalNAc control ASO: CCTTCCCTGAAGGTTCCTCC | Ionis Pharmaceuticals | ISIS-716837 |
| PKCε ASO: GCCAGCTCGATCTTGCGCCC | Ionis Pharmaceuticals | ISIS-1289956 |
| Software and Algorithms | ||
| GraphPad Prism 8.0 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| Other | ||
| Regular chow diet | Envigo | Cat#2018 |
| High-fat diet | Dyets | Cat#112245 |