Table 2.

The normalized %area for quantifying cerebrosides and the analytical performance of GSLs quantification

Normalized %Area for cerebrosides (N=9)
100% of GSLs	NL of Terpy (%, NL215 + NL233)	NL of others* (%, NL18 +N L162 + NL443)	SD
GlcCer(d18:1/18:0)	39.4	60.6	1.2
GalCer(d18:1/18:0)	92.5	7.5	0.2

Analytical Performance
%GlcCer/%GalCer	Avg_Cal %GlcCer	Accuracy (%)**	Inter-day Precision (RSD%)***	Avg_Cal %GalCer	Accuracy (%)*	Inter-day Precision (RSD%)**
90/10	89.9	99.8±0.4	1.0	10.1	101.4±3.9	6.8
50/50	49.3	98.6±2.1	2.2	50.7	101.4±0.9	1.4
10/90	9.3	92.6±4.3	6.7	90.7	100.8±0.5	0.6

^*NL of water, sugar, and Terpy with sphingosine backbone

^**Mean±SD, N=3

^***Relative standard deviation, calculated from three different days with 9 different samples