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. Author manuscript; available in PMC: 2021 Oct 7.
Published in final edited form as: J Am Chem Soc. 2020 Sep 23;142(40):16999–17014. doi: 10.1021/jacs.0c06165

Table 5:

Photophysical properties of thG-labelled (−)/(+) PBS mismatched duplexes

(−)PBS (+)PBS Hypochr omism (%) QY τ1 (ns) α1 f1 τ2 (ns) α2 f2 <τ> (ns) kr (107×S−1) knr (107×S−1)
GthGG CTC 37 0.10 2.4 0.43 0.16 9.3 0.57 0.84 6.3 1.6 14.2
GthGG CAC 13 0.17 4.4 0.39 0.18 12.9 0.61 0.82 9.6 1.8 8.7
GthGG CGC 32 0.23 2.5 0.29 0.05 19.8 0.71 0.95 14.8 1.5 5.2
GthGG CAbC 28 0.15 4.0 0.49 0.25 11.6 0.51 0.75 7.9 1.9 10.8
CthGC GTG 24 0.15 2.1 0.42 0.10 13.5 0.58 0.90 8.7 1.7 9.8
CthGC GAG 15 0.34 2.7 0.19 0.03 18.8 0.81 0.97 15.7 2.2 4.2
CthGC GGG 30 0.21 3.5 0.26 0.08 14.8 0.74 0.92 11.8 1.8 6.7
CthGC GAbG 26 0.12 4.7 0.68 0.42 14.5 0.32 0.58 7.8 1.5 11.2
AthGA TTT 33 0.37 2.3 0.31 0.04 27.3 0.69 0.96 19.6 1.9 3.2
AthGA TAT 12 0.48 2.8 0.22 0.03 28.6 0.78 0.97 22.9 2.1 2.3
AthGA TGT 28 0.42 2.3 0.17 0.02 26.9 0.83 0.98 22.7 1.8 2.6
AthGA TAbT 28 0.47 2.4 0.18 0.02 25.0 0.82 0.98 20.9 2.2 2.5
TthGT ATA 25 0.35 3.0 0.27 0.04 26.8 0.73 0.96 20.4 1.7 3.1
TthGT AAA 25 0.42 3.0 0.24 0.04 23.8 0.76 0.96 18.8 2.2 3.1
TthGT AGA 31 0.33 5.5 0.25 0.09 19.4 0.75 0.91 15.9 2.1 4.2
TthGT AAbA 23 0.25 3.4 0.28 0.07 16.9 0.72 0.93 13.1 1.9 5.7

All reported values are the means for two to four experiments. The standard errors of the mean of the reported values are 8% for the QY, 10% for hypochromism, ± 0.1 ― 0.3 ns for τ1, ± 0.2 ― 0.8 ns for τ2, < 0.05 for the amplitudes (αi) and fractional intensities (fi). The radiative and non-radiative rate constants were calculated as described in Table 1.