| Antibodies |
| Rosetta sequence 1203 designed antibody |
This study |
N/A |
| Rosetta sequence 16996 designed antibody |
This study |
N/A |
| Rosetta sequence 8200 designed antibody |
This study |
N/A |
| Rosetta sequence 7969 designed antibody |
This study |
N/A |
| Rosetta sequence 18444 designed antibody |
This study |
N/A |
| Rosetta sequence 4492 designed antibody |
This study |
N/A |
| Rosetta sequence 11225 designed antibody |
This study |
N/A |
| Rosetta sequence 3083 designed antibody |
This study |
N/A |
| Rosetta sequence 10961 designed antibody |
This study |
N/A |
| Rosetta sequence 17038 designed antibody |
This study |
N/A |
| Rosetta sequence 12374 designed antibody |
This study |
N/A |
| Rosetta sequence 13458 designed antibody |
This study |
N/A |
| Rosetta sequence 9987 designed antibody |
This study |
N/A |
| Rosetta sequence 14857 designed antibody |
This study |
N/A |
| Rosetta sequence 3342 designed antibody |
This study |
N/A |
| CH65 (recombinant CHO-produced IgG1) |
Based on sequence in PDB ID: 5UGY |
N/A |
| CH67 (recombinant CHO-produced IgG1) |
Based on sequence in PDB ID: 4HKX |
N/A |
| CH65:1203d4 Chimera; incorporating Rosetta designed HCDR3 1203d4 into a backbone of CH65 |
This study |
N/A |
| CH65:7969d2 Chimera; incorporating Rosetta designed HCDR3 7969d2 into a backbone of CH65 |
This study |
N/A |
| CH67:1203d4 Chimera; incorporating Rosetta designed HCDR3 1203d4 into a backbone of CH67 |
This study |
N/A |
| CH67:7969d2 Chimera; incorporating Rosetta designed HCDR3 7969d2 into a backbone of CH67 |
This study |
N/A |
| CH65 V106D mutant antibody |
This study |
N/A |
| Anti-influenza nucleoprotein mouse antibodies |
BEI Resources |
Cat# NR-4282 |
| Anti-mouse IgG AP conjugated antibodies |
Novex, DKXMO AP AFFINITY |
Cat# A16014 |
| Goat F(ab′)2 anti-human lambda light chain horseradish peroxidase conjugate |
Southern Biotech |
Cat# 2072-05 |
| Bacterial and Virus strains |
| Influenza A Virus, A/Solomon Islands/3/2006 (H1N1) |
Biodefense and Emerging Infections Research Resources Repository [BEI Resources] |
Cat# NR-41798 |
| Chemicals, Peptides, and Recombinant Proteins |
| 1-Step Ultra TMB-ELISA |
ThermoFisher |
Cat# 34029 |
| Freestyle 293 expression medium |
ThermoFisher |
Cat# 12338002 |
| ExpiCHO Expression Medium |
ThermoFisher |
Cat# A2910001 |
| Fetal Bovine Serum, ultra-low IgG |
ThermoFisher |
Cat# 16250078 |
| Dulbecco’s Modified Eagle Medium |
Invitrogen |
Cat# 11965 |
| HiTrap TALON crude column |
GE Life Sciences |
Cat# 28953766 |
| Deposited Data |
| Crystal structure of apo Fab CH65:7969d2 |
This paper |
PDB: 6DLA |
| Crystal structure of apo Fab CH65:1203d4 |
This paper |
PDB: 6DLB |
| Crystal structure of apo Fab CH67:1203d4 |
This paper |
PDB: 6DL8 |
| Crystal structure of CH65 in complex with SI06 HA |
Whittle et al., 2011 |
PDB ID: 5UGY |
| Crystal structure of CH67 in complex with SI06 HA |
Schmidt et al., 2013 |
PDB ID: 4HKX |
| Crystal structure of apo CH65 |
Lee et al., 2015 |
PDB ID: 4WUK |
| Crystal structure of apo CH67 |
Schmidt et al., 2013 |
PDB ID: 4HKB |
| Experimental Models: Cell Lines |
| EBY-100 yeast cells |
ATCC |
Cat# MYA-4941 |
| Hamster: ExpiCHO-S |
ThermoFisher |
Cat# A29127 |
| Human: FreeStyle 293F |
ThermoFisher |
Cat# R79007
|
| Madin Darby Canine Kidney Cell line |
ATCC |
Cat# CCL-34 |
| Recombinant DNA |
| Heavy-chain BIOMED-2 variable antibody gene-specific primers |
Genscript |
N/A; Custom synthesis |
| TruSeq Library Preparation Kit |
Illumina |
Cat# RS-122-2001 |
| MiSeq PE-300 v3 reagent kit |
Illumina |
Cat# MS-102-3001 |
| Plasmids: Fab heavy chain or lambda chain expression vectors |
Genscript |
N/A; Custom synthesis |
| Software and Algorithms |
| Prism 7 |
GraphPad |
N/A |
| FlowJo 10 |
FlowJo 10 |
N/A |
| UCSF Chimera |
University of California |
N/A |
| PyMOL |
Schrödinger |
N/A; PyMOL version 2.3 |
| Other |
| iQue Screener Plus flow cytometer |
iQue Screener Plus flow cytometer |
N/A |
| Synergy H1 microplate reader |
Synergy H1 microplate reader |
N/A |
| EL406 washer dispenser |
EL406 washer dispenser |
N/A |
| Anti-CH1 CaptureSelect column |
ThermoFisher |
Cat# 19432001L |
| MiSeq instrument |
MiSeq instrument |
N/A |
| Octet RED instrument |
Octet RED instrument |
N/A |
