Fig. 2.

View of fixed Phyllostomus discolor brain, positioned for embedding. In the lower part of the figure the brain is shown in the acrylic glass box used for embedding (cubical volume indicated by fine dotted lines, front and back walls removed). The brain is positioned on 3 pins protruding from the base so that the plane defined by the most dorsal elevation of cerebrum and cerebellum (horizontal tangential plane) as well as the axis through the brainstem, are aligned parallel to the base. The anterior and posterior surfaces of the embedding block define the frontal sectioning plane (sp) perpendicular to the horizontal tangential plane and to the brainstem axis. A pin protruding from a bracket over the front and back walls of the box (only partly shown) prevents brain movement when the embedding medium is poured into the box. In the upper part of the figure the mid-sagittal plane is drawn in a top view of the brain