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. 2020 Sep 25;11:582044. doi: 10.3389/fimmu.2020.582044

FIGURE 7.

FIGURE 7

Serpina3i mRNA expression is up-regulated in mouse lungs upon SplD and IL-33 exposure and decelerates IL-33 degradation in vivo and in vitro. (A) relative mRNA expression of Serpina3i in lungs 6 h after a single i.t. application of PBS or SplD in C57BL/6J, C57BL/6N, DBA/2, CBA, and BALB/c mice as well as BALB/c mice 6 h after IL-33 or SplD + IL-33 i.t. treatment determined by RT-qPCR. Relative mRNA levels determined by RT-qPCR of IL-33 (B) and Il1rl1 (D) determined by RT-PCR. IL-33 protein determined by (C) Luminex and (E) Western blot in mouse lungs of BALB/c mice 12 h after PBS, SplD, SERPINA3I, or SplD + SERPINA3I treatment. Data are presented as mean ± SD. n = 5–9. (F) IL-33 Western blot of recombinant mature murine IL-33 (20 kDa) co-incubated with mMCP-4 for 1 h with and without 1 h preincubation with increasing concentrations of SERPINA3I. One representative blot of three independent experiments is shown. *P < 0.05; **P < 0.01.