Figure 1.

Inhibition of ROCK promotes explant culture in KCM. (A) Schematic representation of the study protocol for explant culture. Human nasal tissues were resected, placed in 8 culture dishes and cultured for 13 days. Cells were collected using trypsin–EDTA and then preserved at − 190 °C. (B) Adhesion rates for explant cultures in KGM containing 0.1 mM Ca²⁺ (0.1 mM Ca²⁺), KGM containing 10 μM Y-27632 (0.1 mM Ca²⁺  + 10 μM Y-27632), KGM containing 1.1 mM Ca²⁺ (1.1 mM Ca²⁺), KGM containing 10 μM Y-27632 and 1.1 mM Ca²⁺ (1.1 mM Ca²⁺  + 10 μM Y-27632), KCM containing 1.6 mM Ca²⁺ (1.6 mM Ca²⁺) and KCM containing 1.6 mM Ca²⁺ and 10 μM Y-27632 (1.6 mM Ca²⁺  + 10 μM Y-27632). Values are expressed as the mean ± SEM (n = 3). (C) Cell confluence in a 60-mm dish for cells cultured in KCM containing 1.6 mM Ca²⁺ (n = 5) or KCM containing 1.6 mM Ca²⁺  + 10 μM Y-27632 (n = 6). (D) Cell number counted from 8 dishes of cells cultured using KCM containing 1.6 mM Ca²⁺ or KCM containing 1.6 mM Ca²⁺  + 10 μM Y-27632. Values are expressed as the mean ± SEM. *P < 0.05, ***P < 0.001.