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. 2020 Oct 8;10:16861. doi: 10.1038/s41598-020-73941-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Settings of the gates for FACS analysis are shown in the upper part of the image. In the lower part of the image, representative images of PBMC isolation, TRAP analysis, DAPI stained cell sorting and results of the resorption assay are presented. The white arrow in the lower right image indicates pit formation in the resorption assay. Yellow points in the DAPI staining mark mononuclear cells whereas red dots mark multinuclear ones.