Figure 5.

(a) Conformational perturbation observed for BSA in response to 0.1–10 µM carprofen (Car), suggesting the binding of carprofen to BSA. Few far-UV CD traces have been truncated beyond certain wavelengths to acknowledge the instrumental limitations. (b) The effect of 1–100 µM carprofen (Car) on the fluorescence spectra of both BSA and ANS. (c) The near-UV CD spectra highlighting the effect of 1–10 µM carprofen (Car) on the tertiary structure of BSA. The CD traces in the presence of 1 and 10 µM carprofen are, respectively, highlighted in blue and red lines.