FIGURE 4.

The differentiation and proliferation of CD4⁺ T cells induced by macrophages. (A) The proliferation index of CD4⁺ T cells induced by macrophages at 72 h after co-culture, measured by MTs kit. (B) Levels of IL-4 production in supernatant of CD4⁺ T cells co-cultured with macrophages. (C) Levels of IFN-γ production in supernatant of CD4⁺ T cells co-cultured with macrophages. CD4⁺ T cells isolated from healthy BALB/c mice co-cultured with macrophages induced by various stimuli, including PBS as blank control (M_PBS), IL-4 as M2 positive control (M_IL–4), INF-γ as M1 positive control (M_INF–γ), BSA as mock control (M_BSA) or rTsTPX2 (M_rTsTPX2). The experimental group PBS representative CD4⁺ T cells dealing with PBS. Statistical analysis was performed with Student’s t-test, and data are presented as mean ± SDs (representative of three experiments). ***P < 0.001, **0.001 < P < 0.01, *0.01 < P < 0.05, NS, not significant. n >= 3 mice per group.