Figure 5.

Reduction of Microglial Activation in Brain and Spinal Cord of SD Mice Treated with AAV9-Bic Vector

(A) Microglial activation was assessed by immunofluorescence staining for CD68 (red) and DAPI to identify nuclei (blue) in brain and spinal cord sections from untreated SD mice (untreated KO), SD mice treated with AAV9-Bic vector (high dose), as well as normal controls (WT). (B) Quantification of CD68 staining intensity showed statistically significant reductions in AAV9-Bic treated mice compared (white bars) to untreated SD mice (black bars) in brainstem and spinal cord (p < 0.05), as well as a trend toward lower levels in thalamus (p = 0.07). No significant differences between groups, including normal controls (gray bars) were apparent in cortex and cerebellum). Abbreviations are as follows: CT, cortex; TH, thalamus; BS, brainstem; CB, cerebellum; SC, spinal cord. Scale bars, 150 μm. Two tailed t tests were used to determine statistical significance (∗p < 0.05).