Figure 4.

Detection Sensitivity of T4NT CAR T Cells

(Top) PET images of indicated CAR T cell numbers acquired 15 minutes after radiolabeling with [¹⁸F]BF₄⁻ (see Materials and Methods). Typical images are shown from one T4NT batch. (Bottom) Cumulative analysis of obtained imaging data from three T4NT batches (error bars are SD). Dashed line indicates background signals, with gray shade indicating background mean ± three times SD. The limit of detection was defined as background mean plus three times SD of the background mean and is marked by the upper dotted line. Cell detection limit is ~3,000 T4NT CAR T cells.