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. 2020 Jun 24;28(10):2252–2270. doi: 10.1016/j.ymthe.2020.06.025

Figure 6.

Figure 6

IL-6 Expression Is Induced by Lymphodepleting Chemotherapy

(A and B) pSTAT3 expression was determined by flow cytometry among splenic myeloid cells collected from NT or LD mice treated with or without ex vivo IL-6 stimulation. (A) Representative histogram with mean fluorescence intensity (MFI) values indicated adjacent to each histogram. (B) Summarized data from a representative experiment. (C) Representative histogram showing IL-6R expression among BM CMPs and GMPs from NT or LD mice at day 1 after lymphodepletion. (D) Percentage of IL-6R+ CMPs and GMPs at day 1 and day 7 after lymphodepletion. (E) Experimental design for (F)–(H). Bone marrow was collected from NT or LD B16-bearing mice and cultured for 24 h. (F) Stacked bar chart of intracellular IL-6 expression by CD11b+ myeloid cells, Lin−c-kit+ HSPCs, lymphocytes (CD3+/CD19+/NK1.1+), and CD45−Lin− cells as determined by flow cytometry. (G) Representative histograms showing IL-6 expression among individual BM cell subsets with or without ex vivo LPS stimulation. MFI is indicated on the right of each histogram. (H) Fold change in IL-6 expression among individual BM cell subsets. In (A)–(D), data are representative of two to three independent experiments. In (H), normalized data are a compilation of three independent experiments with biological replicates shown. Data were normalized to NT or NT+LPS. p values were determined by a two-tailed Student’s t test. In (B) and (D), errors bars represent SEM. In (H), error bars represent SD.