Skip to main content
. 2020 Sep 25;11:566584. doi: 10.3389/fphys.2020.566584

FIGURE 1.

FIGURE 1

Ouabain suppresses IL-6 signaling in cultured human myotubes by reducing the abundance and the phosphorylation of STAT3. (A–D) Human myotubes were treated with or without 50 nM ouabain (OUA) in Advanced MEM with 2% FBS for 20 h and in serum-free Advanced MEM for 4 h. Cells were treated with tocilizumab (100 μg/ml, TCB) during the last 4 h and with interleukin-6 (50 ng/ml, IL-6) during the last 15 min. (E–L) Human myotubes were treated in serum-free Advanced MEM with different concentrations of OUA for 24 h (E–H) or with 50 nM OUA for 1–24 h (I–L). (A,E,I) α1-subunit of Na,K-ATPase (NKAα1), (B,F,J) the total STAT3 and (C,G,K) phospho-STAT3 (Tyr705) were measured by immunoblotting. Graphs in panels (D,H,L) show the phospho-STAT3:STAT3 ratio. Results are means with SEM (n = 4–8). *p < 0.05 vs. Basal.