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. 2020 Sep 25;11:566584. doi: 10.3389/fphys.2020.566584

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Copyright © 2020 Pirkmajer, Bezjak, Matkovič, Dolinar, Jiang, Miš, Gros, Milovanova, Pirkmajer, Marš, Kapilevich and Chibalin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ouabain does not suppress STAT3 and HIF-1α in rat L6 myotubes. L6 myotubes were treated in serum-free MEMα with or without 50 nM ouabain (OUA) for 24 h and with or without 250 μM CoCl₂ during the last 4 h. (A) α1-subunit of Na,K-ATPase (NKAα1), (B) the total STAT3, (C) phospho-STAT3 (Tyr705), (D) HIF-1α, (E) phospho-Src (Tyr527), (F) the total ERK1/2, (G) phospho-ERK1/2 (Thr202/Tyr204), (H) phospho-p70S6K (Thr389), (I) the total S6RP, (J) phospho-S6RP (Ser235/236), (K) the total 4E-BP1 and (L) phospho-4E-BP1 (Thr37/46) were measured by immunoblotting. Results are means with SEM (n = 3). *p < 0.05 vs. Basal.