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. 2020 Sep 25;11:570661. doi: 10.3389/fimmu.2020.570661

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Copyright © 2020 Hwang, Silva-Sanchez, Carragher, Garcia-Hernandez, Rangel–Moreno and Randall.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The presence of iBALT does not affect Th2 priming or accumulation in the lung. (A) Timing of iBALT induction, allergic sensitization and analysis. (B,C) The frequencies (B) and numbers (C) of EGFP⁺CD4⁺ T cells were determined by flow cytometry. (D) Timing of iBALT induction, transfer of naïve OTII cells, allergic sensitization, antigen challenge and analysis. (E,F) The frequency (E) and number (F) of donor T cells in the lungs were determined by flow cytometry. All data show mean ± SD of 5 mice per group; **P < 0.01, ***P < 0.001. Experiments were performed 2 times (A–C) or 3 times (D–F).