FIGURE 4.

Little induction of apoptotic DCs by A. faecalis. (A) BMDCs were co-cultured without (None) or with live A. faecalis or E. coli at 10 MOI for 24 or 48 h, after which Annexin V⁺, 7AAD^– apoptotic cells were detected by FACS. (B) BMDCs were co-cultured with live E. coli at 10 MOI for 48 h in the absence or presence of iNOS inhibitor SEIT and then apoptotic cells were detected by FACS. (C) BMDCs were co-cultured with live A. faecalis at 10 MOI for 48 h in the absence (None) or presence of A. faecalis LPS or E. coli LPS at 0.1 μg/ml and then apoptotic cells were detected by FACS. The results shown in (A,C) are representative of two independent experiments. The result shown in (B) is combined from two independent experiments. *P < 0.05; **P < 0.01; n.s., not significant, which is described in Materials and Methods.