Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 8;11:5077. doi: 10.1038/s41467-020-18916-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020, corrected publication 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a, b Identifying infiltrated cell types in BC and non-malignant tissues. a Workflow of the sample preparation, sequencing and bioinformatic analysis. b tSNE plot of single cells profiled in the presenting work colored by major cell types, tumor grade and patient. c–h Reclustering of EPCAM+ cells. c UMAP plot EPCAM+ cells (epithelial marker) colored by cluster, patient, grade and CNV level. d Heatmap of differentially expressed genes (DEGs) of every CNV group. e Enriched GO functions of downregulated genes in malignant cells. f Expression levels of MHC-II molecules and CD74. g Immunofluorescence (IF) staining of MHC-II molecules and EPCAM. Scale bar represents 50 μm. h Heatmap shows difference in pathway activities scored by GSVA per cell between different CNV groups. Shown are t-values from a lineal model.