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. 2020 Oct 8;3:556. doi: 10.1038/s42003-020-01285-6

Fig. 5. MSR1 interacts with CHKV nsP1.

Fig. 5

a, b Co-immunoprecipitation (co-IP) of FLAG-Msr1 (mouse) with Myc-tagged CHIKV proteins expressed in HEK293 cells using a mouse anti-FLAG antibody (IP), followed by immunoblotting (IB) with a rabbit anti-FLAG and anti-Myc antibody. WCE: whole-cell extract. N.S: non-specific bands. E: CHIKV envelope, C: capsid, P: non-structural protein. c co-IP of FLAG-MSR1 (human) with Myc-nsP1 in HEK293. d Immunofluorescence staining for endogenous MSR1 and CHIKV nsP1 in trophoblasts without (mock) / infected with CHIKV at a multiplicity of infection of 0.5 for 12 h. MSR1 and nsP1 were stained by a rabbit anti-MSR1 and rat anti-nsP1, followed by an Alexa Fluor-594 (red) and -488 (green) conjugated secondary antibody respectively. The cells were counterstained for nuclei by DAPI (blue). Scale bar: 20 µM. The yellow punctae in the overlay indicate co-localizations. e Co-IP of FLAG-MSR1 fragments with endogenous nsP1 in trophoblasts infected with CHIKV (MOI = 0.5 for 12 h) using a mouse anti-FLAG antibody (IP), followed by IB with a rabbit anti-FLAG and anti-nsP1 antibody. Vec: vector. Mock: mock infection. The uncropped immunoblots for all figures can be found in Supplementary Fig. 3.