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. 2020 Oct 8;10:16846. doi: 10.1038/s41598-020-73913-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Dynamic experiment showing the variation of the chewed gum microbial communities in a twelve weeks period. (a) Alpha diversity shows the richness of the samples based on the Richness, Shannon, and Simpson index, (b) Clustered-Bar chart represents the modification of the oral bacterial profile over time, according to 16S rRNA gene monitoring. (c) Beta diversity (PCoA) based on Bray–Curtis illustrates correlations among the bacterial genera in different samples. Distances to the linear statistical correlation and the colours indicate the similarity of the microbial communities affected by environmental factors or removing time during the three-month period of the experiment.