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. 2020 Oct 8;10:16789. doi: 10.1038/s41598-020-74026-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Quantitative RT-PCR on human Caco-2 cells stimulated with Vitamin D and PFOA. Relative gene expression of Transient Receptor Potential Cation Channel Subfamily V Member (TRPV6), Calbindin-D9k (CaBP9K) and Cytochrome P450 Family 24 Subfamily A Member 1 (CYP24A1) after 24 h stimulation with 1,25-dihydroxyvitamin D (VD, grey bars), PFOA (dotted bars) or both (VD + PFOA, black bars), normalized to the expression of the housekeeping GAPDH on control (CTRL, no stimulation, white bars). Data are shown as the mean ± standard deviation (SD) of three different experiments performed in triplicate. (B) Quantitative RT-PCR on human SAOS-2 cells stimulated with Vitamin D and PFOA. Relative gene expression of osteocalcin (OCN), p21 (CDKN1A), Cytochrome P450 Family 24 Subfamily A Member 1 (CYP24A1) and Calbindin-D9k (CaBP9K) after 24 h stimulation with 1,25-dihydroxyvitamin D (VD, grey bars), PFOA (dotted bars) or both (VD + PFOA, black bars), normalized to the expression of the housekeeping GAPDH on control (CTRL, no stimulation, white bars). Data are shown as the mean ± standard deviation (SD) of three different experiments performed in triplicate. *p < 0.05 vs. CTRL; #p < 0.05 vs. VD.