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. Author manuscript; available in PMC: 2020 Oct 9.
Published in final edited form as: Cell Rep. 2020 Sep 1;32(9):108086. doi: 10.1016/j.celrep.2020.108086

Figure 6. Model of Mutations Necessary for Efficient In Vitro OSE-SC Transformation.

Figure 6.

Random mutagenesis assays and targeted experiments revealed minimal requirements for adhesion-independent growth and mutations that enhance transformation. The blue box contains genes that are minimal requirements for transformation or cause transformation at low efficiency. The addition of mutations shown in the yellow box cause significant degrees of transformation. The addition of further mutations in genes shown in the green box allow for the highest rates of transformation. Genes listed in the red box inhibit transformation. However, two exceptions exist. Brca2 and Fancd2 (marked with a single asterisk) co-mutagenesis alongside Trp53 and Rb1 result in efficient OSE-SC transformation. Similarly, Rad51c (marked with two asterisks) and Fat3 plus Trp53, Cdkn2a, and Pten caused efficient transformation.