KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-TRP53 | Cell Signaling | Cat#9282 |
| Rabbit polyclonal anti-β-actin | Abcam | Cat#ab8227 |
| Goat anti rabbit HRP | Cell Signaling | Cat#7074S |
| Rat monoclonal anti CK8 (TROMA-1) | University of Iowa Developmental Hybridoma Bank | Cat#AB_531826 |
| Alexa Fluor 488 goat polyclonal anti rat antibody | Thermo Fisher | Cat#A-11006 |
| Bacterial and Virus Strains | ||
| One Shot Stbl3 competent E. coli | ThermoFisher | Cat#C737303 |
| LentiCRISPRv2 | Addgene | Cat#52961 |
| FUGW | Addgene | Cat#14883 |
| Biological Samples | ||
| Trp53+/− FVB/NJ Mouse Ovaries | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Cas9 mRNA | TriLink | Cat#L-7206-20 |
| Phosphate-Buffered Saline | Thermo Fisher | Cat#10010023 |
| 0.25% trypsin EDTA solution | Thermo Fisher | Cat#25200072 |
| DMEM | VWR | Cat#10-017-CM |
| Fetal Bovine Serum (FBS) | Atlanta Biologicals | Cat#S11050H |
| Collagenase | Sigma-Aldrich | Cat#10269638001 |
| Dispase | Sigma-Aldrich | Cat#10269638001 |
| DNaseI | Sigma-Aldrich | Cat#11284932001 |
| Bovine Serum Albumin (BSA) | Sigma-Aldrich | Cat#A9418 |
| Gelatin from porcine skin | Sigma Aldrich | Cat#G9136-10MG |
| Hams F12 | Thermo Fisher | Cat#11320033 |
| Hydrocortisone | Sigma-Aldrich | Cat#H4001 |
| Insulin-transferrin-sodium selenite | Sigma-Aldrich | Cat#11074547001 |
| Non essential amino acids (NEAA) | Thermo Fisher | Cat#11140050 |
| Glutamate | Thermo Fisher | Cat#25030081 |
| Sodium pyruvate | Thermo Fisher | Cat#11360070 |
| Penicillin-streptomycin | Thermo Fisher | Cat#15140122 |
| RIPA buffer | Sigma Aldrich | Cat#R0278-50ML |
| 4-15% gradient polyacrylamide gels | BioRad | Cat#4561083EDU |
| Nitrocellulose membranes | Thermo Fisher | Cat#88018 |
| BSMBI restriction enzyme | New England Biolabs | Cat#R0580S |
| T4 DNA ligase | New England Biolabs | Cat#M0202S |
| Ampicillin | Sigma-Aldrich | Cat#A0166 |
| LB broth | Sigma-Aldrich | Cat#L3147 |
| Methanol | Sigma-Aldrich | Cat#322415-1L |
| Goat serum | Sigma-Aldrich | Cat#NS02L |
| Coverslip mounting media | Vector Laboratories | Cat#H-1000 |
| TransIT-LT1 transfection reagent | Mirus | Cat#MIR 2305 |
| Amicon Ultra-15 columns | Millipore | Cat#UFC903024 |
| 0.45um syringe filters | Thermo Fisher | Cat# 725-2545 |
| Puromycin | Sigma-Aldrich | Cat#8833-10MG |
| Agarose | VWR | Cat#VWRVN605-500G |
| Critical Commercial Assays | ||
| MEGAshortscript T7 Transcription kit | Thermo Fisher | Cat#AM1334 |
| MinElute Columns | QIAGEN | Cat#28004 |
| BCA assay | Thermo Fisher | Cat#23227 |
| GeneJet Plasmid Miniprep kit | Thermo Fisher | Cat#K0502 |
| GeneJet Plasmid Midiprep kit | Thermo Fisher | Cat#K0481 |
| ALDEFLUOR detection kit | StemCell Technologies | Cat#01700 |
| Agencourt DNAdvance DNA isolation kit | Beckman Coulter | Cat#A48705 |
| QiAquick PCR purification kit | QIAGEN | Cat#28104 |
| Surveyor mutagenesis assay | Integrated DNA Technologies | Cat#706020 |
| Cell Biolabs Inc 96 Well Cell Transformation Assay | Cell Biolabs | Cat#CBA-135 |
| Miseq Reagent Kit v2 | illumina | Cat#MS-102-2001 |
| Experimental Models: Cell Lines | ||
| HEK293T | ATCC | CRL-3216 |
| HELA | ATCC | CCL-2 |
| OSN2 | Alexander Nikitin | Corney et al., 2007 |
| Experimental Models: Organisms/Strains | ||
| FVB/NJ Mice | Jackson Laboratories | Stock#001800, MGI:2163709 |
| Oligonucleotides | ||
| See Tables S1–S3 | ||
| Recombinant DNA | ||
| 60 unique LentiCRISPRv2 constructs | This paper | N/A |
| LentiCRISPR v2 | Addgene | Cat#52961 |
| PsPax2 | Addgene | Cat#12260 |
| VSV-G | Addgene | Cat#8454 |
| FUGW | Addgene | Cat#14883 |
| Software and Algorithms | ||
| BWA MEM software | arXiv:1303.3997 | N/A |