Fig. 4.

TREM-1 expression is up-regulated by CM from NLRP3 inflammasome-activated macrophages through ROS-NF-κB signaling. A: The protocol for conditioned medium experimentation. Macrophages were treated with ROS scavenger NAC (100 μM) for 30 min, then exposed to 20% CM-LA. Fluorescence density of mitoROS was measured in macrophages treated with CM-CON, CM-LA, or CM-LA + MCC950 for 3 h (B, n = 3). Trem-1 mRNA expression was determined by real-time PCR (C, n = 3). TREM-1 (D-E, n = 3), p-IκB, and IκB (F-I, n = 3) protein expressions were detected by western blotting 24 h later. The data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, and *** P < 0.001.