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. 2020 Oct 7;219(11):e202002160. doi: 10.1083/jcb.202002160

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© 2020 Pedersen et al.

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Figure 2. — Lifetimes and relative fluorescence intensities of GFP-tagged query proteins agree with prior results but reveal inherent variability. (A) Box-and-whisker plots of maximum fluorescence intensity of the indicated GFP-tagged query protein imaged with reference to Ede1-RFP (left), Sla1-mCherry (center), and Abp1-RFP (right). (B) Box-and-whisker plots of lifetimes of the indicated GFP-tagged query protein when imaged with reference to Ede1-RFP (left), Sla1-mCherry (center), and Abp1-RFP (right). Red lines are median values, boxes indicate interquartile range, and whiskers indicate full range of the data. Colored text indicates which module each protein imaged belongs to according to the color scheme below.