Figure S5.

Defining molecular components of the early and late steps of the endocytic pathway. (A) Maximum-intensity projections of spinning disk confocal z-stacks of clusters of cells endogenously expressing Sla1-mCherry (magenta) and Hrr25-3xGFP (green, top), Apl1-GFP (green, second row), Pal1-GFP (green, third row), or Ent1-GFP (green, last row). Individual channels are shown in grayscale at left. Images are representative of at least three separate experiments. (B) Maximum-intensity projections of epifluorescence z-stacks of clusters of 7Δ cells endogenously expressing Sla1-GFP (green) and Abp1-mCherry (magenta). Individual channels are shown in grayscale at left. (C) Quantification of the ratio of the number of Abp1-mCherry sites to the number of Sla1-GFP sites in small-budded (Sm) and large-budded (Lg) wild-type and 7Δ cells (n = 30 cells per category). Numbers are P values from Kruskal–Wallis tests followed by Dunn’s multiple comparisons test. The median and interquartile ranges are denoted with error bars.