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. 2020 Sep 29;14:1177932220962106. doi: 10.1177/1177932220962106

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Pan-genome size of NC S. pneumoniae isolates: (A) Proportions of gene coding sequences in the pan-genome divided into 4 categories: the hard-core and the soft-core genome, representing respectively 99% and 95% to 99% of genes shared between isolates. The shell genes are present in 15% to 95% of isolates, whereas the cloud genes are distributed in less than 15% of isolates.^29,30,49 (B) Cumulative number of genes in the pan-genome. Box plots indicate first and third quartiles with medians shown as horizontal lines.