Figure 1.

Evaluation of drug toxicity and efficacy in SOD1 G93R mutant fish. (A) Scheme describing the procedure undertaken to evaluate drug toxicity and efficacy in mSOD1 larvae. Toxicity evaluation included acute toxicity, such as apoptosis/necrosis, specific organ toxicity (liver, kidney, heart, etc), cardiovascular system abnormalities (heart rate, morphology, hemorrhage and edema) and behavioral toxicity. Efficacy was evaluated by analyzing locomotor ability and endurance. (B) 6 dpf WT and mSOD1 larvae were subjected to dark/ light transition. The distance they swam after light stimuli per 1 min time bin was measured and averaged. (***P < 0.001; linear mixed model; n = 72 per each group). (C) mSOD1 larvae were treated with vehicle (0.1% DMSO; Ct) or 1 µmol/L Riluzole. The distance they swam per 1 min time bin after light stimuli was measured and averaged. (***P < 0.001; linear mixed model; n = 72).