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. 2020 Sep 11;7(10):1883–1897. doi: 10.1002/acn3.51174

Figure 5.

Figure 5

Cipro/Celecox preserved the ramified morphology of surveillant microglia. Morphological analysis of individual microglia in the tectum of zebrafish larvae. (A–C) 3D reconstructed Apotome z‐stack images of branching Apo‐E:GFP microglial cells. (A'–C') The backbone (colored processes) of microglia cells traced with the Filaments analysis of Imaris software. (A, A') WT. (B, B') mSOD1 control treated with vehicle solvent. (C, C') mSOD1 treated with 100 µmol/L Cipro/ 1 µmol/L Celecox. (Scale bar = 10 µm). (D–H) Morphometric analysis measurements of individual microglia in WT, mSOD1 control and mSOD1 treated with Cipro/Celecox. Graphs indicate (D) convex hull area, (E) number of branch points, (F) process length, (G) number of segments and (H) full branch level. (ns = non significant; *P < 0.05; **P < 0.01; ***P < 0.001; one‐way ANOVA, Tukey post hoc test, n = 30 for WT fish n = 20 for control mSOD1, n = 17 for Cipro/Celecox treated mSOD1 fish).