Table 1.

Dox concentration in plasma and peritoneal cavity cells 60 min after i.v. bolus administration of 15 mg/kg

Parameter	Animal groups
	Control	'Cure' TBM	'Relapse' TBM	'Resistance' TBM
Plasma Dox (ng/µl)	0.25±0.050	0.25±0.066	0.22±0.054	0.21±0.049
Cellular PerC Dox (ng/106 cells)	11±0.6	11±0.5	9±0.4*	7±0.8*
Cellularity PerC (n × 106)	2.2±0.30	2.3±0.30	2.8±0.21	24.2±0.40**
EL4 cell number in PerC (n × 106) or tumor mass (mg) (#)	N.A.	0.2±0.03	0.6±0.08*	22.5±0.31**
EL4 cellular density in PerC (106/ml)	N.A.	0.6±0.14	1.8±0.96*	26.8±3.6**
Cellular Dox (dMCF) in EL4 cells (##)	N.A.	85±2.2	58±1.7*	11±2.5**
Animal body weight (g)	20.0±0.87	19.9±0.43	19.9±0.95	20.3±0.70
Ratio (%) of calculated tumor mass to animal weight	N.A.	0.001	0.003	0.045
Cellular Dox AUC(0-10d) (µg/g × h)	8.3±0.49	8.2±0.57	4.6±0.27*	1.6±0.02**

*P < 0.05; **P < 0.01;

N.A. - not applicable Dox - doxorubicin; PerC - peritoneal cavity; TBM - EL4 tumor bearing mice; dMCF - difference in mean channel fluorescence; AUC - Area under the concentration curve;

#: Tumor mass calculations were made from the EL4 percentage values and PerC cellularity. One mg of tumor without ascitic fluid component equals 1 × 10⁶ cells;

##: EL4 gated cellular fluorescence increase (mean channel volume) 60 min after Dox i.v. bolus administration.