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. 2020 Sep 23;11(22):6623–6632. doi: 10.7150/jca.46562

Figure 5.

Figure 5

DSCAM-AS1 contributes to CRC cell proliferation and migration by targeting miR-137/Notch-1 axis. A-C: The efficiency of Notch-1 overexpression was analyzed by RT-qPCR and western blotting. D: The efficiency of miR-137 inhibition was analyzed by RT-qPCR. E: Western blotting was applied to test the protein expression of Notch-1. F: Quantification of protein expression of Notch-1. G: Cell proliferation assay (CCK-8) was used for the examination of cell proliferation ability in CRC cells transfected with different plasmids. H: The migration ability of transfected cells was measured by transwell assay. I: Immunofluorescence staining of vimentin and E-cadherin in HT29 cells. *P < 0.05.