Figure 5.

Downregulation of UPK1A inhibits HCC cell glycolysis via regulating HIF-1α signaling. (A) GSEA analysis indicated that UPK1A positively correlated with gene signature involved in hypoxia and HIF-1α signaling. (B) mRNA expression level of UPK1A and HIF-1α after silencing UPK1A in SK-Hep-1 cell were detected using qRT-PCR. (C) HIF-1α expression level after silencing UPK1A in SK-Hep-1 cell was evaluated using western blot. (D) Expression level of HIF-1α target genes after silencing UPK1A in SK-Hep-1 cell were detected using qRT-PCR.