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. 2020 Sep 2;16(15):2883–2894. doi: 10.7150/ijbs.45710

Figure 4.

Figure 4

The p38-MAPK/HSP27 signalling pathway was involved in dioscin-induced apoptosis. (A) NCI‐H520 and SK‐MES‐1 cells were treated with dioscin (5 µM) for 48 h. The levels of p-p38, p38, p-HSP27 and HSP27 were analysed by western blotting. (B, C) NCI‐H520 and SK‐MES‐1 cells were treated with Anisomycin (0.5 µM) for 24 h. Then, cell apoptosis was measured by flow cytometry, and the expression levels of p-p38, p38, p-HSP27, HSP27, Bax, Bcl2, cleaved caspase-3 and cleaved PARP were analysed by western blotting. (D) NCI‐H520 and SK‐MES‐1 cells were pretreated with SB203580 (5 µM) for 2 h before exposure to dioscin for 48 h. Then, cell apoptosis was measured by flow cytometry. Data are presented as the mean ± SD of triplicate samples. *P < 0.05, **P < 0.01, and ***P < 0.001 vs control.