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. 2020 Sep 19;16(15):2974–2988. doi: 10.7150/ijbs.50186

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The identification and verification of NcoR1-dependent genes. (A) The heat map of differentially expressed genes dependent on NcoR1. Total RNA samples from RAW246.7 (Control), NcoR1-KD1 and NcoR1-OE cells were used for microarray analysis. The aberrantly expressed genes that showed converse expression patterns in NcoR1-KD1 and NcoR1-OE cells were selected and presented in a heat map. (B-G) RT-qPCR results. Total RNA samples isolated from Control-KD, NcoR1-KD (#1 and #2), Control-OE, and NcoR1-OE cells were used to detect the mRNA levels of six genes, HMGB1 (B), IL6 (C), CCL2 (D), DNM2 (E), FEN1 (F), and CCN2 (G). * P < 0.05, ** P < 0.01 and *** P < 0.001.