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. 2020 Sep 25;11:558712. doi: 10.3389/fimmu.2020.558712

Figure 2.

Figure 2

Inhibition of PKR activity decreases NLRP3 inflammasome priming/activation in LPS/ATP challenged fibroblasts. Fibroblasts were pretreated with the PKR inhibitor (C16) for 30 min. Subsequently, the fibroblasts were challenged with LPS (1 μg/mL) for 6 h followed by ATP (3 mM) for 30 min. (A–C) NLRP3 and pro-IL-1β protein levels were indices of inflammasome priming. Representative Western blots are shown in (A) and densitometric analyses in (B,C). (D–F) Caspase 1 activation (p20) and release of mature IL-1β were indices of inflammasome activation. Representative Western blot for caspase 1 p20 is shown in (D) and densitometric analysis in (E). Protein levels of IL-1β were assessed in supernatants by ELISA. n = 3, *p < 0.05 as compared with control; #p < 0.05 as compared with LPS+ATP.