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. 2020 Sep 25;7:586450. doi: 10.3389/fmolb.2020.586450

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Copyright © 2020 Teng, Jiang, Huang, Guo, Chen, Hou, Xi and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cy5-labeled RecQ exhibits the same dsDNA unwinding activity as that of wild-type RecQ. (A) Schematic representation of the smFRET experiment DNA (10-nt overhang). DNA was surface immobilized using a biotin–streptavidin bridge onto a polyethylene glycol passivated coverslip. Cy3 signal is lost when Cy3-labeled 3′ overhang strand is unwound by RecQ. (B) Representative field of view before and after unwinding by 5 nM RecQ-Cy5 and 2 mM ATP. Each white dot represents fluorescent DNA molecules. (C) Fractions of unwound DNA molecules on coverslip vs. time in indicated reaction conditions. Error bars represent standard deviation (SD) from three experiments.